Перегляд за автором "Zaiets, I.V."

Результати 1-3 з 3

Generation of HEK-293 stable cell lines with disrupted expression of ribosomal protein S6 kinase (S6K1) isoforms using the CRISPR/Cas9 genome editing system

Zaiets, I.V.; Sivchenko, A.S.; Khoruzhenko, A.I.; Filonenko, V.V. (Вiopolymers and Cell, 2017)

Aim. To generate HEK-293 cells with disrupted expression of S6K1 isoforms: p85, p70 and p60. Methods. CRISPR/Cas9 gene editing, Western blotting, immunofluorescent analysis, RT-PCR analysis, MTT assay, scratch assay. ...
Identification of a novel S6K1 splice variant coding for the p60-S6K1 isoform

Zaiets, I.V.; Holiar, V.V.; Filonenko, V.V. (Вiopolymers and Cell, 2019)

Aim. To identify a splicing mRNA of S6K1 kinase specific for the p60-S6K1 isoform alone. Methods. RT-PCR, DNA sequencing, Western blotting. Results. RT-PCR analysis of total RNA extracted from MCF-7 cells and subsequent ...
Optimization of in vitro model for analysis of tumor cell migration dynamics

Kravchenko, A.O.; Kosach, V.R.; Shkarina, K.A.; Zaiets, I.V.; Tykhonkova, I.O.; Khoruzhenko, A.I. (Вiopolymers and Cell, 2018)

Migration ability is an important feature of tumor cells. There are several approaches to analyze the dynamics of cancer cell migration in vitro. One of the most perspective and closer to the in vivo conditions is the model ...

Результати 1-3 з 3

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